溶血对16项生化指标的影响及其结果的校正系数的探讨

Study on the impact of hemocytolysis on sixteen biochemical indicators and the correction factors for the measurement

目的:探讨不同溶血程度对常用16项生化指标的影响,并对溶血与生化指标相关性进行回归分析。方法:采用HITACHI7600-010全自动生化分析仪测定200份标本溶血前后的16项生化指标,以基础血清为对照,进行相关性分析,观察结果的变化与结果回归的可能性。结果:溶血对常规生化指标的GOT,TP,ALB,TG,CKMB,HDBH,LDH,K+有明显的相关性,并产生正干扰,对TBIL,DBIL,IDBIL、GLU的相关性及产生的干扰与溶血的程度相关,并有明显的临界性,对GSP中度相关,AKP、GPDA负相关,而对LP-a相关性较低。结论:溶血对不同生化检测指标的干扰机制不同,不同溶血程度对生化常规指标产生不同的影响,不同生化指标的检测结果应采用不同回归处理。

Objective：To investigate the impact of hemocytolysis on 16 biochemical indicators and the corrective measures.Methods： Sixteen serum biochemical indicators were detected in 200 blood samples before and after hemocytolysis by using HITACHI 76O0-010 auto-biochemical analyzer and basal serum was used as a control.Correlation analysis was used to evaluate changes in biochemical indicators and possibility of regression.Results： Hemocytolysis has significantly correlation of positive interference with routine biochemical indicators GOT,TP,ALB,TG,CKMB,HDBH,LDH and K＋;the correlation of interference between hemocytolysis and TBIL,DBIL,IDBIL,GLU is criticality related to the degree of hemocytolysis;hemocytolysis also has medium correlation with AKP,GSP,CKMB,GPDA,and low correlation with LP-a.Conclusion： Our studies demonstrated that hemocytolysis in different degree has different influence on routine biochemical indicators due to the differences in mechanism of action;the use of regression analysis can enhance the accuracy of test results.