响应面优化超声提取苦荞麦苗黄酮的研究(英文)

Optimization of Ultrasound-assisted Extraction of Flavonoids from Tartary Buckwheat Seedling by Response Surface Methodology

研究苦荞麦苗黄酮超声提取方法。采用三因素五水平的星点设计响应面分析研究影响苦荞麦苗黄酮超声提取条件。最佳提取条件为:乙醇体积分数76.2%、液固比28.5:1(mL/g)、超声时间25min,在最佳提取条件下,苦荞麦苗黄酮提取率为3.64%。结果表明,超声提取具有简单、低成本和高效的特点,可有效改善总黄酮的提取,苦荞麦苗可作为健康食品和黄酮原料具有较好的应用前景。

Ultrasound-assisted extraction for the flavonoids-enriched extract from tartary buckwheat seedling was investigated. Response surface methodology, based on the central composite design of three variables with five levels, was employed to study the effect of extraction conditions on the extraction yield of total flavonoids. An experimental extraction yield of 3.64% was achieved under the optimum extraction conditions of 76.2% ethanol volume fraction, liquid/solid ratio of 28.5：1 and extraction time of 25 min. This study confirms the efficiency of ultrasound-assisted extraction as a simple, inexpensive and effective method to improve the extraction of flavonoids and the great potential of tartary buckwheat seedling as a health food and a resource of flavonoids.