响应面法优化闪式提取牛蒡苷的工艺优化

Optimization of Homogenate Extraction for Arctiin from Arctium lappa L. by Response Surface Methodology

采用响应面分析法优化牛蒡苷闪式提取的工艺条件。以牛蒡苷得率为指标,在单因素试验基础上,应用Box-Benhnken中心组合设计试验,对提取工艺影响较大的乙醇体积分数、提取时间和液料比3个工艺参数进行优化。最佳工艺条件为:乙醇体积分数83%、提取时间93s、液料比17:1(mL:g)、牛蒡子粒径≤1.0mm,在此工艺条件下,牛蒡苷得率为31.62mg/g,与预测值31.72mg/g基本相符。闪式提取法是一种快速有效的提取牛蒡苷的方法。

Response surface analysis （RSA） methodology was used for optimizing homogenate extraction processing of arctiin from Arctium lappa L. Based on single-factor experiments, the effects of ethanol concentration, extraction time and liquid-material ratio on extraction rate of arctiin were explored by Box-Benhnken central composite design method. The optimal extraction conditions were ethanol concentration of 83%, extraction time of 93 s, liquid-material ratio of 17：1 （mL/g） and particle size of less than 1.0 mm. Under these optimal extraction conditions, the actual yield of total arctiin was 31.62 mg/g, which was consistent with the predicted yield of 31.72 mg/g. Results indicated that homogenate extraction was an effective method to extract arctiin from Arctium lappa L.