万寿菊花中反式叶黄素提取皂化工艺的优化

Extraction and Saponification of Trans-lutein from Marigold Flowers

以万寿菊干花颗粒为原料,对万寿菊花中反式叶黄素进行同时提取皂化工艺研究。考察不同有机溶剂和用量、KOH-乙醇溶液质量浓度和用量、提取皂化温度和时间等因素对反式叶黄素提取皂化效果的影响。在单因素试验基础上,采用响应曲面法对影响万寿菊花中反式叶黄素提取皂化效果的3个主要因素即石油醚用量、KOH-乙醇溶液质量浓度和提取皂化温度进行优化,建立并分析各因素与反式叶黄素得率关系的数学模型,同时对反式叶黄素粗品进一步分离纯化。结果表明:采用有机溶剂法进行同时提取皂化的最佳工艺条件为石油醚用量42.6mL/g、温度58.8℃、KOH-乙醇溶液质量浓度0.099g/mL,在此条件下反式叶黄素得率为1.499%。对反式叶黄素粗品进一步纯化,最终获得纯度达90.42%的反式叶黄素晶体。

Marigold （Tagetes erecta） flowers were used as raw materials to investigate the extraction and saponification of trans-lutenin from marigold flowers. The effects of organic solvent amount, KOH-ethanol concentration and amount, extraction and saponification temperature and time on extraction and saponification rate of trans-lutenin from marigold flowers was explored. Based on the single factor experimental results, response surface experiments were used to optimize the extraction and saponification efficiency of trans-lutein and a mathematic model between the factors and extraction rate of trans-lutenin from marigold flowers was established. Meanwhile, crude trans-lutein was further purified. Results indicated that the optimal extraction parameters were raw material amount of 1.00 g, petroleum ether amount of 42.6 mL/g, KOH-ethanol concentration of 0.099 g/mL and extraction temperature of 58.8 ℃. Under the optimal extraction conditions, the extraction rate of trans-lutein was up to 1.499%. After the purification of trans-lutein, the content of trans-lutein crystal was up to 90.42%.