水溶性大豆多糖的提取工艺研究

Extraction Processing of Soluble Soybean Polysaccharides

以脱脂豆粕为材料,对豆粕中水溶性大豆多糖的提取、纯化进行研究。通过酶提取效果选择,在木瓜蛋白酶、碱性蛋白酶、复合蛋白酶3种酶中选取提取效果最佳的碱性蛋白酶;通过从提取溶液的pH值、提取时间、料液比、酶的添加量及提取温度等因素的试验分析比较,得出大豆多糖的最优提取条件。结果表明:当提取溶液pH值、提取温度、提取时间和液料比分别为6.0、50℃、1.5h和20:1(mL/g)时,豆渣中大豆多糖的提取率可达到最大值17.92%,粗多糖的纯度为86.32%。

Soybean meal was used as the experimental subject to explore the extraction and purification of soluble soybean polysaccharides （SSPS）. During the choice of enzyme, alkali protease exhibited the best enzymatic extraction efficiency among papain, alkali protease and complex protease. The optimal extraction conditions of SSPS were explored by evaluating the effects of extraction pH, extraction time, material-liquid ratio, enzyme addition amount and extraction temperature on extraction rate. Results indicated that the optimal extraction conditions were extraction pH of 6.0, extraction temperature of 50 ℃, extraction time of 1.5 h and material-liquid ratio of 1：20. Under these optimal extraction conditions, extraction rate of SSPS was up to 17.92 % and the purity of SSPS was 86.32%.