金针菇水溶性多糖物理提取工艺及优化

Optimization of Extraction Processing for Water-soluble Polysaccharides from Flammulina velutipes

对比研究湿法超微粉碎提取、微波辅助提取、超声波辅助提取等不同提取方法,对金针菇可溶性多糖提取效果的影响,采用响应曲面试验设计法优化湿法超微粉碎提取金针菇水溶性多糖的工艺条件。结果表明,湿法超微粉碎提取法能显著提高金针菇水溶性多糖的提取率,优化后的工艺参数为胶体磨磨齿间隙42μm、提取温度87℃、提取时间170min。根据模型预测提取液中多糖含量为417.6μg/mL,验证实验中多糖含量为(418±3.21)μg/mL,提取率为3.63%。

Wet ultra-fine pulverization extraction, microwave-assisted extraction and ultrasound-assisted extraction were used to explore the optimal extraction conditions of water-soluble polysaccharides from Flammulina velutipes. Response surface methodology （RSM） was employed to optimize the conditions of wet ultra-fine pulverization extraction for water-soluble polysaccharides. Results indicated that the optimal extraction processing parameters were the gap of colloid mill at 42μm, extraction temperature of 87 ℃ and extraction time of 170 min. Under the optimal extraction conditions, the extraction rate of polysaccharides was 3.63%. The concentration of water-soluble polysaccharides based on predictive model was 417.6μg/mL, and the actually determined concentration of water-soluble polysaccharides was （418 ± 3.21 ）μg/mL.