蓝莓类饮料中花青素的快速检测方法

Rapid Detection Methods for Anthocyanins in Blueberry Beverage

目的:建立蓝莓饮料中飞燕草定-3-O-葡萄糖苷及锦葵定-3-O-葡萄糖苷两种花青素类成分的高效液相色谱快速检测方法。方法:采用高效液相色谱法,使用安捷伦ZORBAXSB-C18(4.6mm×75mm,3.5μm)色谱柱,以3%磷酸溶液和甲醇为流动相,二极管阵列检测器在波长525nm处进行检测。结果:该方法对飞燕草定-3-O-葡萄糖苷和锦葵定-3-O-葡萄糖苷的最低检出限分别为0.4ng和2.5ng,标准曲线线性良好,相关系数大于0.9984,回收率在90.2%～98.3%之间,相对标准偏差不大于1.79%。结论:该法简洁、快速且灵敏度高,适合于蓝莓类饮料中花青素的快速检测。

Objective： To establish a high performance liquid chromatography （HPLC） method for simultaneous determination of delphinidin-3-O-glucoside and malvidin-3-O-glucoside in blueberry beverage. Methods： The anthocyanins including delphinidin-3-O-glucoside and malvidin-3-O-glucoside were determined by HPLC with ZORBAX SB-C18 （4.6 mm × 75 mm, 3.5μm） as the chromatographic column, 3% phosphoric acid-methanol as the mobile phase, diode array as the detector and detection wavelength at 525 nm. Results： The detection limits of this HPLC method for delphinidin-3-O-glucoside and malvidin-3-O-glucoside were 0.4 ng and 2.5 ng, respectively. The standard curve determined by this method exhibited an excellent linear relationship with correlation coefficient larger than 0.9984. The recovery rate of this method was in the range of 90.2%-98.3% with relative standard deviation less than 1.79%. Conclusion： The established HPLC method is a simple, rapid and sensitive determination method, which is suitable for the rapid determination of anthocyanins in blueberry beverage.