红曲橙色素对人胃腺癌细胞AGS诱导凋亡的作用

An orange Monascus pigment induces apoptosis on human gastric adenocarcinoma AGS cells

经分离纯化获得红曲霉发酵产品中的一种橙色素,通过HPLC-MS和NMR检测,确认为Monascorubrin,分子组成为C23H26O5.体外实验表明,该色素组分能有效抑制人胃癌细胞AGS的增殖,IC50值为7.98±0.71μmol·L-1.5μmol·L-1药物处理24h,93.70%AGS细胞进入凋亡前期.细胞凋亡程度与药物浓度呈正相关关系.该色素组分对AGS细胞生长抑制效果显著优于紫杉醇(P<0.01),且对人正常胃上皮细胞(GES-1)的毒性作用较紫杉醇小(P<0.05).

An orange pigment was purified from Monascus products,and it was identified as Monascorubrin（C23H26O5） by HPLS-MS and NMR.The experiments in vitro demonstrated that the target compound was toxic to human gastric adenocarcinoma cell line AGS with IC50 value of 7.98±0.71 μmol·L-1.93.70% of AGS cells was in the early phase of apoptosis when treated with 5 μmol·L-1 Monascorubrin for 24 h.The treatment exhibited a marked dose-dependent inhibition.Compared with taxol,Monascorubrin showed a higher growth inhibition（P 0.01） on AGS cells but less cytotoxicity effect（P 0.05） on normal GES-1 cell.