疏肝理气法对功能性消化不良大鼠下丘脑和胃窦胃泌素、生长抑素表达的影响

Effect of method of soothing liver and regulating qi on expression of gastrin and somatostatin in hypothalamus,castric antrum of functional dyspepsia rats

目的:观察疏肝理气法对实验性功能性消化不良大鼠下丘脑和胃窦胃泌素、生长抑素表达的影响。方法:32只大鼠随机分为正常组、模型组、柴胡疏肝散组、吗丁啉组,每组8只,以夹尾激惹刺激法制作功能性消化不良大鼠模型,并分别给予生理盐水、柴胡疏肝散水煎剂、吗丁啉溶液灌胃,免疫组化方法检测各组大鼠下丘脑和胃窦胃泌素、生长抑素的表达。结果:模型组大鼠下丘脑和胃窦GAS表达均较正常对照组显著降低(P<0.05,P<0.01),下丘脑和胃窦SS表达均较正常对照组明显增加(P<0.01);柴胡疏肝散组和吗丁啉组胃窦GAS,SS表达均较模型组分别上调和下调,差异均具有显著性(P<0.05,P<0.01),吗丁啉组下丘脑GAS,SS表达较模型组均无显著性差别,柴胡疏肝散组下丘脑GAS表达较模型组显著上调,而SS表达则与模型组无显著差别。结论:疏肝理气法能上调中枢和外周GAS表达、下调外周胃组织SS表达,可能是其有效治疗FD的作用机制之一。

Objective： To explore the effects of the method of soothing the liver and regulating qi on expression of gastrin and somatostatin in hypothalamus and gastric antrum of functional dyspepsia model rats.Method： The 32 rats were randomly divided into normal group,model group,Chaihu Shugansan group and domperidone group（n=8）.The functional dyspepsia model was established by constantly squeezing their tails and mean while saline,Chaihu Shugansan decoction and domperidone suspension were administered respectively to 4 groups by gavage.The expression of gastrin and somatostatin in hypothalamus and gastric antrum of rats by immunohistochemical were detected 3 weeks later.Result： The expression of GAS in the hypothalamus and gastric antrum of model group were less than those of normal group（P〈0.05,P〈0.01）,while the expression of SS in the hypothalamus and gastric antrum in Model group were significantly increased than those of normal group（P〈0.01）.The expression of GAS and SS in gastric antrum of Chaihu Shugansan group and domperidone group were increased and decreased respectively,and the differences were significant（P〈0.05,P〈 0.01）.There were no obvious difference about expression of GAS,SS in the hypothalamus between domperidone group and model group.GAS expression in hypothalamus of Chaihu Shugansan group were increased than those of normal group but there was no obvious difference in SS expression in hypothalamus between Chaihu Shugansan group and model group.Conclusion： The method of soothing the liver and regulating qi can increase GAS expression in central and peripheral and decrease SS expression in peripheral gastric antrum,which may be one of its therapeutic mechanisms on functional dyspepsia.