蓬莱松组织培养研究(简报)

STUDY ON TISSUE CULTURE OF Asparagus myrioculadus

以蓬莱松（Ａｓｐａｒａｇｕｓｍｙｒｉｏｃｕｌａｄｕｓ）的嫩枝为外植体，在ＭＳ基本培养基附加不同浓度的植物生长调节物质的培养基上于室温２５±２℃、光照１４ ｈ／ｄ、光强１５００ ｌｘ 条件下进行组织培养，结果表明，ＭＳ附加６ＢＡ ０．２～０．５ ｍ ｇ／Ｌ可促进转接的愈伤组织分化芽，附加６ＢＡ ０．２～０．５ｍ ｇ／Ｌ或结合ＮＡＡ ０．１～０．２ ｍ ｇ／Ｌ，对蓬莱松嫩芽增殖、生长亦具有良好的促进作用，每芽可增殖４～９倍。

The resultsofexperimentindicated thatcalliwere induced from young stalk explantsofAsparagusmyrioculadus,on MSm edia containing 0.2,0.5 and 1.0 m g/L6 BA, the bud differentiation from calliw as prom oted on MSm edia containing 0.2 and 0.5 m g/L 6 BA,In proliferation media,0.2 and 0.5 m g/L 6 BA alone or com bined with 0.2 m g/L NAA were of great advantage to bud propagation and growth of young shoots,the bud propagation rates were 400% ～900% 。