采用低氧训练方法建立大鼠心肌组织血管新生动物模型

Using Hypoxia Training Methods to Establish an Animal Model of Angiogenesis of Atrial Tissue of SD Rats

目的:研究低氧训练对大鼠心肌组织血管生成的作用,从而构建大鼠心肌组织微血管新生的动物模型。方法:将健康雄性SD大鼠60只,按体重随机分为6组,每组10只,对照组,高住组,常练组,低住高练组,高住低练组,高住高练组。采用递增负荷跑台运动训练及低氧作为处理因素,建立大鼠低氧训练模型。采用免疫组织化学、显微图象分析对心肌组织毛细血管密度、光密度水平、表达面积进行计数和检测。结果:CD34能很好地标记心肌组织微血管,低氧训练对心肌组织微血管的生成效果理想。结论:采用低氧训练方法可以建立大鼠心肌组织血管新生的动物模型,为血管的生理性重建提供新的研究方法和途径。

Objective： This paper is to study the hypoxic training on angiogenesis of atrial tissue of SD rats,and establish a model of animal training for angiogenesis of atrial tissue of SD rats.Methods： 60 male SD rats were randomly divided into six groups,10 rats each,which are normoxic control group,hypoxic living group,normoxic training group,normoxic living hypoxic training group,hypoxic living normoxic training group,and hypoxic living hypoxic training group.Progressive treadmill exercise and hypoxia are used to establish hypoxic training animal model.Using the immunity histochemistry and the microimage analysis to count and check the capillary density、opitical density level、expression area of atrial tissue.Results： It turns out that the CD34 can mark microvascular of atrial tissue very well,and hypoxic training has get a satisfactory result in angiogenesis.Conclusions： We conclude that hypoxic training can be established an animal model of angiogenesis of atrial tissue of SD rats,and it can provide new research ways and means for physiological reconstruction of blood vessels.