安胃汤对CAG模型大鼠TFF2 mRNA表达的影响

Effect of Anwei decoction on expression of TFF2 mRNA in CAG model rats

目的探讨复方中药安胃汤对实验性慢性萎缩性胃炎大鼠模型TFF2mRNA表达的影响。方法采用甲基硝基亚硝基胍(MNNG)慢性萎缩性胃炎大鼠模型,36只清洁级健康雄性Wistar大鼠随机分为病理模型组、安胃汤组、胃复春对照组共3组。实时定量PCR方法检测各组胃黏膜TFF2mRNA/β-actin值,观察复方中药安胃汤对实验性慢性萎缩性胃炎大鼠模型胃黏膜TFF2mRNA表达的影响,并行统计学分析处理。结果与病理模型组相比,复方中药安胃汤组和胃复春对照组均能显著提高TFF2mRNA/β-actin相对表达量的比值(0.90±0.06vs0.35±0.06和0.72±0.10vs0.35±0.06,P<0.01),复方中药安胃汤组优于胃复春对照组(0.90±0.06vs0.72±0.10,P<0.05)。结论安胃汤可能通过增加胃黏膜TFF2mRNA表达,增强受损胃黏膜周围完好的上皮细胞向黏膜损伤表面迁移覆盖或与黏液中的糖蛋白相互作用,从而增强胃肠道黏膜防御屏障的保护能力,达到防治CAG的目的 。

Objective To explore the effect of Anwei decoction（AD）on the expression of TFF2 mRNA in rats with chronic atrophic gastritis（CAG）.Methods Thirty-six healthy rats were divided randomly into three groups.Group A was treated without any medicine,group B was treated with AD and group C was treated with medicine Weifuchun.CAG in all of the rats in each group was induced by N-methy-N-Nit ro-N-Nitroguanidine（MNNG）.The effect of medicines on the expression of TFF2 mRNA in rat＇s gastric mucosa was determined by real-time polymerase chain reaction（real-time PCR）.Results The expressions of TFF2 mRNA were remarkably enhanced in group B（0.90±0.06）and C（0.72±0.10）in comparing to that in group A（0.35±0.06,P0.01）,and the difference of TFF2 mRNA expression between group B and C of was statistically significant（P0.05）.Conclusions Anwei decoction treats CAG possibly by enhancing the expression of TFF2 mRNA in gastric mucosa,which might improve the integrity and defensive ability of gastrointestinal mucosal.