Rho A和Ezrin蛋白在鼻咽癌组织中的表达及临床意义

Significance of Rho A and Ezrin expression in nasopharyngeal carcinoma

目的探讨RhoA和Ezrin蛋白表达在鼻咽癌浸润、转移及预后等方面中的作用及其相互关系。方法用Super-visionTM免疫组化方法检测RhoA和Ezrin蛋白在150例不同临床分期的鼻咽癌组织及20例鼻咽黏膜慢性炎组织中的表达情况。结果①RhoA和Ezrin蛋白在鼻咽癌组织中的阳性表达率分别为62.7%和74.7%,显著高于黏膜慢性炎组(P均<0.001)。②RhoA和Ezrin蛋白表达与鼻咽癌临床分期特别是T分期有关,差异具有统计学意义(P<0.01或P<0.05)。③鼻咽癌组织中RhoA和Ezrin蛋白表达呈显著正相关(r=0.486,P=0.000)。④RhoA和Ezrin单独或联合表达阳性患者放疗后中位生存时间短于阴性患者(P<0.05)。结论 RhoA和Ezrin蛋白表达与鼻咽癌的浸润、转移有密切关系,在鼻咽癌浸润、转移过程中起到协同作用。二者单独或联合表达提示预后不良。

Objective To investigate the expressions of protein Rho A and Ezrin in nasopharyngeal carcinoma（NPC）tissue at different clinical stages,and to analyze their functions and relationship in NPC pathogenesis.Methods Paraffin-embedded tissues from 150 cases of NPC with complete clinical data were enrolled as experiment group,and the tissues from 20 cases of nasopharyngeal mucosa chronic inflammation（NMCI）were enrolled as compare group.Immunochemical staining were applied to detect the expressions of protein Rho A and Ezrin.Results The positive expression rates of Rho A and Ezrin in NPC tissue were 62.7% and 74.7% respectively,which was significantly higher than those in NMCI tissues（P0.001）.The positive expression rates of Rho A and Ezrin in NPC tissue were significantly related to TNM stages（P0.05 and P0.001,respectively）.The expressions of Rho A and Ezrin in NPC were significantly correlated（r=0.486,P=0.000）.Patients with Rho A and/or Ezrin positive expression had significantly shorter median survival time after radiotherapy than the patients with negative expression（P0.05）.Conclusion The expressions of Rho A and Ezrin protein are closely related to NPC invasion and metastasis,in which they might have synergistic effect.Rho A and Ezrin expressed alone or in combination indicates a poor prognosis of NPC.