RP-HPLC法检测酒花中的黄腐酚、异黄腐酚与8-异戊烯基柚皮素

Simultaneous Determination of Xanthohumol,Isoxanthohumol and 8-Prenylnaringenin in Hop using RP-HPLC

为实现酒花中3种有效组分——黄腐酚、异黄腐酚和8-异戊烯基柚皮素的同步检测,利用反相高效液相色谱建立这3种物质的同步检测体系。采用ODS C18色谱柱(250 mm×4.6 mm,4μm),乙腈-1%甲酸溶液作流动相,非线性梯度洗脱,流速0.8 mL/min,柱温30℃,紫外检测波长370 nm和288 nm。试验结果表明,在优化的色谱条件下,3种物质的标准曲线线性关系良好,相关系数均大于0.998,精密度好(RSD<1%),回收率在93%～106%之间。该检测方法可以同步测定酒花中的黄腐酚、异黄腐酚和8-异戊烯基柚皮素。此外,考察了不同溶剂的提取效果,结果发现用中等极性溶剂提取这3种物质的效果较好。

In order to achieve simultaneous determination of xanthohumol,isoxanthohumol and 8-prenylnaringenin in hop,a method based on RP-HPLC was developed in this study.An ODS C18 column（250 mm×4.6 mm,4 μm） with temperature 30 ℃ was used.Acetonitrile-1% formic acid solution were used as mobile phase at a flow rate of 1.0 mL/min with a nonlinear gradient elution program.The detection wavelengths were controlled at 370 nm and 288 nm.Results showed that under the optimized chromatographic condition,good linearity was obtained for all of these three components with correlation coefficients exceeding 0.998,recoveries ranged between 93% and 106%,while the precision was 1%.The evaluation parameters indicate that this method can accurately determine the content of xanthohumol,isoxanthohumol and 8-prenylnaringenin in hop.In addition,the effect of different extraction solvents was investigated using the developed method and some middle-polar solvents had been found having good extraction performance with high content of these three components.