新疆树上干杏不定芽再生影响因子的研究

Factors Affecting Shoot Regeneration of Apricot Cultivar "SHU shang-gan"

以新疆树上干杏茎段为材料,研究了外植体消毒、植物激素的种类及其浓度和取材时间等因素对茎段不定芽再生的影响。结果表明:75%酒精30 s+0.1%HgCl25 min+15%H2O220 min具有较好的灭菌效果,且不会较大影响茎段不定芽的再生;最适诱导不定芽再生的培养基为改良MS+TDZ 1.0 mg/L+NAA 0.1 mg/L,培养15 d后出芽率可达到90.8%;分裂素TDZ促进不定芽再生的效果优于6-BA;春季取材效果明显优于夏季取材。

Factors affecting successful and rapid shoot regeneration of apricot cultivar ＂SHU shanggan＂ was studied.Ethanol,HgCl2 and H2O2 were applied in several combinations for disinfection;time of disinfection,plant growth regulators TDZ,6-BA and NAA,season of obtain the explants were evaluated for the optimization of shoot regeneration.The results showed that 30 s 75% Ethanol＋5 min 0.1% HgCl2＋20 min 15% H2O2 was the best combinations for disinfection,The highest number of new microshoots was obtained in the culture medium,modified MS＋1.0 mg/L TDZ＋0.1 mg/L NAA,with 90.8% shoot regeneration rate.We found that TDZ was better than 6-BA in adventitious shoot regeneration and we＇d better take the explants in spring.