胼胝兜兰的组培快繁技术研究

Study on Tissue Culture and Propagation Techniques of Paphiopedilum callosum

胼胝兜兰种子在自然条件下萌发困难,自然更新能力差。现采用人工授粉的种子,进行了非共生萌发研究。结果表明:270 d胚龄的种子萌发率高(>95%);种子萌发最佳培养基为1/2RE+CM 100 mL/L+椰粉12 g/L+香蕉泥70 g/L+蔗糖20 g/L+琼脂粉5 g/L;壮苗培养基以MS+NAA 1.0 mg/L+BA 0.4 mg/L+椰粉12 g/L+香蕉70 g/L+蔗糖30 g/L+琼脂粉5 g/L为好,小苗用松树皮移栽效果好,移栽成活率95%以上。

The seeds of Paphiopedilum callosum were difficult to germinate under field conditions,which with poor natural regeneration.Investigation of asymbiotic germination were carried out on the seeds of artificial pollination.The results showed that 270-day-embryo-aged-seeds had the highest germination rate;The best medium for germination was 1/2RE＋coconut milk 100 mL/L＋coconut meat 12 g/L＋mashed banana 70 g/L,The strong seeding medium was MS＋NAA 1.0 mg/L＋BA 0.4 mg/L.Pine bark was best for seeding cultivation.The study achieved rapid propagation of Paphiopedilum callosum and it could advance the propagation coefficient,which provide the basis for commercially produce.