IGF-1对SD大鼠骨髓间充质干细胞成骨成脂方向分化的影响

Effect of IGF-1 on the adipogenic or osteogenic differentiation of BMSCs in SD rat

目的：观察胰岛素样生长因子-1（IGF-1）作用于骨髓间充质干细胞后核心结合因子α1（CBFα1）和过氧化物酶增殖物激活受体γ2（PPARγ2）基因表达情况,探讨其对骨髓间充质干细胞成脂方向分化的影响。方法：用贴壁法筛选分离纯化SD大鼠骨髓基质干细胞并传代培养,取第4代SD大鼠骨髓间充质干细胞作为实验样本,随机分为空白对照组、低剂量IGF-1（10μg/L）组与高剂量IGF-1（20μg/L）组进行干预培养12 h,采用RT-PCR法检测各组CBFα1和PPARγ2 mRNA的表达。结果：IGF-1高剂量组及低剂量组均可以提高大鼠骨髓间充质干细胞CBFα1的mRNA以及下调PPARγ2的mRNA表达,高、低剂量组与空白对照组差异均有统计学意义（P均〈0.05）,但是这种剂量-效应在IGF-1干预浓度介于10~20μg/L的情况下并不明显。结论：IGF-1使骨髓间充质干细胞PPARγ2基因表达水平降低,阻碍骨髓间充质干细胞向成脂方向分化;使CBFα1基因表达水平增高,促进BMSCs成骨方向分化。IGF-1可能参与骨髓间充质干细胞成骨成脂方向分化的调节,并有利于成骨方向分化。

Objective：To investigate the impact of IGF-1 on CBFα1 and PPARγ2 gene expression in bone marrow mesenchymal stem cells（BMSCs）,and explore the impact of IGF-1 on the ability of the adipogenic or osteogenic differentiation in BMSCs.Methods：BMSCs from SD rats were isolated and purified by differential attachment method.The forth generation of SD rats bone marrow stromal cells were randomly divided into control group,low doses group with IGF-1 group（10 μg/L） and high doses group（20 μg/L）.All underwent intervention culture for 12 hours,and mRNA of CBFα1 and PPARγ2 were determined by RT-PCR.Results：IGF-1 at low or high does up-regulated the expression level of CBFα1 and down-regulate the expression of PPARγ2.There was significant difference between low,high-dose group and control group.But this dose-dependent effect was not obvious at 10 μg/L to 20 μg/L.Conclusions：IGF-1 can down-regulate PPARγ2 gene expression,increase CBFα1 gene expression,impede adipogenic ability of BMSCs,then promote osteogenic differentiation in BMSCs.IGF-1 may involve in regulation of the adipogenic or osteogenic differentiation in BMSCs,and facilitate osteogenic differentiation.