芪蚣抗纤方活血化瘀药对肝纤维化大鼠血液粘稠度的影响

Effects of Qigongkangxian formula on blood viscosity in rat models of hepatic fibrosis

目的：探讨芪蚣抗纤方活血化瘀药抗肝纤维化的作用机理。方法：40只雄性SD大鼠随机分为正常对照组（N组）、模型组（M组）及芪蚣抗纤方活血化瘀低、高剂量组（HD组、HG组）,除N组外,其余3组猪血清腹腔注射法复制肝纤维化大鼠模型,HD、HG组分别用1 mL/200 g体重、2 mL/200 g体重活血方灌胃,用药4周后,比较各组脾重、脾重指数及血液粘稠度。结果：M组脾重、脾重指数及全血高、中、低切粘度、血浆粘度均明显高于N组（P〈0.05~0.01）,给予活血化瘀药后,各指标较M组明显降低（P〈0.05~0.01）,HG组较HD组改变更明显。结论：芪蚣抗纤方活血化瘀药能通过改善肝脾微循环,降低血液粘度,减轻瘀血程度,有保护肝细胞,改善肝纤维化的作用。

Objective：To explore the mechanism of anti-fibrosis function of Qigongkangxian formula.Methods：Forty male SD rats were randomly divided into control group（group N）,cross-type group（group M）,low and high dosage Qigongkangxian formula group（group HD,group HG）.Except group N,the other 3 groups were made models of hepatic fibrosis by intraperitoneal injection of porcine serum,besides,group HD and HG were also treated with intragastric administration of 1 mL/200g and 2mL/200g weight,respectively.Four weeks later,spleen weight,spleen weight index and blood viscosity were evaluated and compared among these groups.Results：Comparing with group N,group M showed significant heavier spleen weight,higher spleen weight index and blood viscosity at high,medium and low shear（P0.05-0.01）,while after treatment of drugs for invigorating blood circulation and eliminating blood stasis all indexed in group M decreased significantly.Changes after treatment were more significant in group HG comparing with group HD.Conclusions：Qigongkangxian formula can decrease blood viscosity,invigorate blood circulation,protect hepatic cells thus to improve hepatic fibrosis.