大豆质膜内在水孔蛋白的生物学功能预测

Prediction of Biological Function of Plasma Membrane Aquaporins in Soybean

[目的]利用生物信息学方法对大豆质膜内在水孔蛋白(GmPIP)的亚细胞定位、跨膜区、信号肽和磷酸化位点进行预测,为基因的功能研究提供参考。[方法]从干旱胁迫后的抗旱大豆品种晋豆21的叶片中克隆出GmPIP基因,利用PSLpred、Protcomp Version8.0、WoLF PSORT、CELLOv2.5等亚细胞预测工具进行亚细胞定位预测;利用TMHMMServerv.2.0进行蛋白跨膜区分析;利用NetPhos2.0 Server进行磷酸化位点分析;利用SignalP3.0 Server程序进行信号肽分析。[结果]综合4种工具的预测结果表明,GmPIP蛋白包含6个跨膜区,亚细胞定位预测表明该蛋白位于细胞质膜上,发现该蛋白的第254个氨基酸是一个潜在的糖基化位点和13个磷酸化位点,该蛋白没有明显的信号肽结构。[结论]利用不同方法进行的生物信息学分析结果可相互验证,生物信息学可以对未知蛋白进行初步的功能预测,有助于确定后续试验的方向。

[Objective] This study aims to predict the sub-cellular localization,Transmembrane,signal peptide and phosphopeptides of plasma membrane aquaporins in soybean GmPIP.[Method]GmPIP gene was cloned from soybean cultivar jindou 21.Software for the prediction was used such as PSLpred,Protcomp Version8.0,WoLF PSORT,CELLO v2.5 for sub-cellular localization.Procedure TMHMM Server v.2.0,NetPhos2.0 Server and SignalP 3.0 Server were used to predict transmembrane,signal peptide and phosphopeptides respectively.[Result] There are 6 transmembrane domains and 13 phosphopeptides in GmPIP,and it was predicted to localize on the plasma membrane,and No signal peptide was found.[Conclusion]The results of the bioinformatics analysis offer a good suggestion for the function analysis of GmPIP next.