响应面设计法优化牡丹皮中总酚的提取工艺

Optimization of Extracting Total Phenolic Compounds from Cortex Moutan by Response Surface Methodology

[目的]利用响应面设计法优化牡丹皮(CortexMoutan)中总酚的提取工艺。[方法]固定提取时间30 min,以乙醇体积分数、料液比、提取温度为响应因子,以总酚的提取率为考察指标,采用3因素3水平的响应面分析法优化总酚的醇提工艺参数。[结果]牡丹皮中总酚的最佳提取工艺条件为:乙醇体积分数50%,提取温度75℃,料液比1∶41。在此优化条件下,牡丹皮总酚的提取得率为40.95mg/g,这与模型预测值相符。[结论]该研究可为牡丹皮的深入开发和广泛应用提供科学依据。

[Objective]To optimize the extraction process of total phenolic compounds from Cortex Moutan [Method]Extraction time of 30 min,ethanol concentration,extraction temperature and solid-liquid ratio were chosen as the response factors,the extraction rate of total phenolic compounds as index.The best combination of extraction parameters was obtained by response surface methodology at a three-variable and three-level experiment.[Result]The optimum extraction parameters were as follows：50% ethanol,extraction temperature of 75 ℃,solid-liquid ratio of 1∶41.Under the above conditions the maximal yield of phenolic compounds reached 40.95 mg/g,which was agreed with model predictions.[Conclusion]The study would provide scientific proof for the deeper utilization and extensive application of Cortex Moutan