水稻条纹叶枯病抗性基因SSR标记的筛选及应用

Screening and Application of SSR Markers of Resistance Gene against Rice Stripe Virus

[目的]设计和筛选新的SSR引物,以用于水稻条纹叶枯病抗性改良的回交育种中。[方法]以高抗条纹叶枯病晚粳品种502,高感条纹叶枯病晚粳品种秀水09等常规晚粳品种及其衍生株系为材料,在利用SSR和SARP标记对高抗条纹叶枯病RSV1基因定位的基础上,进一步设计3对(M-11-1,M-11-2,M-11-3)SSR标记。通过筛选和分析,选择M-11-3作为检测RSV1基因的标记基因用于追踪RSV1基因,从而实现将RSV1基因导入秀水09等晚粳品种的回交育种中,鉴定各个株系的抗性表现。[结果]改良系的条纹叶枯病抗性明显高于秀水09,绝大部分的抗性接近供体水平,而且在不同年份间抗性表现稳定,因此试验中利用的RSV1基因抗性效应十分明显,与RSV1基因连锁标记M-11-3辅助选择准确。[结论]研究证明了分子标记用于水稻条纹叶枯病抗性改良的可行性,同时也表明,优化和发展新的标记基因能够有效提高标记辅助选择的效率。

[Objective]New SSR primers were designed and screened to apply in the backcross breeding for modified resistance against rice stripe virus.[Method]The conventional late japonica rice varieties including 502 with high resistance to stripe virus,Xiushui09 with high susceptibility to stripe virus and their derived strains were adopted as the test material,SSR and SAPR markers was used to locate RSV1 gene with high resistance against stripe virus,and three pairs of SSR markers（M-11-1,M-11-2,M-11-3） were further designed.Through screening and analysis,M-11-3 was selected as the RSV1 detection marker gene for tracking RSV1 gene,thus RSV1 gene was successfully introduced to the backcross breeding of late japonica rice varieties such as Xiushui 09,and the resistance expression of different strains was identified.[Result]The resistance of improved strains against stripe virus was significantly higher than Xiushui 09,the resistance of most strains was close to the level of donor,and the expression of resistance among years was stable.Therefore,the resistance effect of RSV1 gene used in the test was very obvious,which was accurate with the assisted selection of RSV1 gene linked markers M-11-3.[Conclusion]The study certified the feasibility of molecular markers application in resistance improvement against rice stripe virus,which also showed that optimization and development of new marker genes could effectively improve the efficiency of marker-assisted selection.