几种植物提取液对感染TMV烟草叶片核酸及类黄酮含量的影响

Effect of Several Kinds of Extracts from Plant on the Content of Nucleic Acid and Flavonoid in the Tobacco Leaf Infested by TMV

[目的]研究几种提取液对烟草花叶病毒(TMV)的抑制作用及其防治效果,为植物源抗病毒类生物农药的研制提供依据。[方法]以K326为试验材料,采用常规摩擦接种,感染病毒后,喷施白花蛇舌草、莲提取液及由20种植物加工而成的植物源抗病毒制剂(配伍制剂),于接种后第5、10、15、20天测定核酸及类黄酮含量,分析几种提取液对TMV的抑制作用。[结果]喷施植物提取液后,烟株体内的DNA含量升高,均高于阳性对照,说明烟株细胞恢复了正常的合成能力;而RNA含量比阳性对照低,说明烟株体内的TMV含量降低,喷施植物提取液能够恢复植物细胞的正常功能;类黄酮含量增加。植物提取液抑制了TMV在烟株体内的复制能力,其中以配伍制剂的效果最好。[结论]喷施植物提取液能够恢复植物细胞的正常功能,以喷施配伍制剂的效果最好。

[Objective] The theoretical basis of the preparation of the plant type of bio-agrochemical agent resisting tobacco mosaic virus（TMV） was provided through the research on the inhibition and controlling effect of several extracts from the plant on TMV.[Method] The tobacco——K326,infected by TMV through the conventional mechanical method,was sprayed with the bio-agrochemical agents prepared made of the extract from 20 species of plants such as Hedyotis diffusa and so on.The content of nucleic acid and flavonoid was tested in the tobacco leaf on the 5th,10th,15th and 20th day after its being infested and the inhibition role of the extract to TMV was analyzed.[Result] The content of DNA in plant after its being treated was increased compared with the CK,which indicated the normal synthesis of the cell was recovered.The content of RNA was decreased compared with the CK,which indicated the content of TMV in plant was decreased and the treatment could promote the function of plant cell being normal again.The content of flavonoid was also increased after treatment.[Conclusion] The treatment of bio-agrochemical agent resisting TMV could inhibit the copy of it in tobacco plant.