广东省狂犬病病毒分离株N基因的序列分析

Sequence Analyses on N Gene of Rabies Viruses Isolated from Guangdong Province

[目的]确定广东地区狂犬病毒流行毒株,更好地防控狂犬病。[方法]对2009年从广东省各地采集的犬脑和对应唾液腺进行直接免疫荧光和巢式RT-PCR检测,并通过颅内接种乳鼠进一步鉴定毒株。PCR扩增分离株的N基因,测定其核苷酸序列,并将其与广西地区的流行毒株和疫苗株进行了比对。[结果]分离到2株狂犬病毒毒株,其N基因序列与广西分离到的病毒具有极高的相似性(97.9%～99.4%),与狂犬病毒疫苗株的相似性为87.7%～88.1%。[结论]外表健康犬只可能携带狂犬病毒,且狂犬病毒的流行存在明显的地域差异。

[Objective] To find the dominant rabies virus strains in Guangdong Province and thus to better prevent and control rabies.[Method] Canine brains and salivary glands collected in several regions of Guangdong Province were detected by direct immunofluorescence assay and nested RT-PCR.The isolates were further identified by intracranial inoculation in suckling mice.The N gene was amplified by RT-PCR and the sequences were aligned with those of prevalent strains in Guangxi region and vaccine strains.[Result] Two strains were isolated from Guangdong Province.Their N gene sequences had high similarity of 97.9%-99.4% to those of Guangxi strains and also had similarity of 87.7%-88.1% to those of vaccine strains of rabies virus.[Conclusion] Apparently healthy dogs may carry rabies virus,and the prevalence of rabies virus shows obvious regional differences.