免疫营养剂对胃癌癌前病变的调理作用

Effects of Immunonutrition in Precancerous Lesions of Gastric Cancer in Rats

目的:研究免疫营养制剂对大鼠胃癌癌前病变阶段的影响。方法:制造大鼠胃癌癌前病变的模型,90只大鼠随机分为对照组、模型组和实验组,模型组采用普通的饲料喂养,实验组在普通的饲料喂养的基础上添加免疫加强营养制剂瑞能[(120ml/(kg·d)]。分别在造模成功后4,8,12周显微镜下观察鼠胃黏膜的变化,AgNOR染色观察细胞的增殖和分化,并抽血检测IgG、IgA、IgM及T淋巴细胞亚群CD3+、CD4+和CD8+T细胞。结果:处于癌前病变阶段的大鼠胃黏膜表面部分上皮变性、坏死和脱落,并可见肠上皮化生。经过喂养免疫营养后,显微镜下表现接近于正常组表现。模型组较正常鼠有不同程度的免疫力下降,IgG升高,IgA、IgM较正常对照组明显下降(P<0.05),CD4+/CD8+T细胞比值下降(P<0.05);喂养免疫加强营养制剂12周后,IgG下降,IgA、IgM恢复正常,CD4+/CD8+比值上升(P<0.05);实验组胃黏膜上皮细胞AgNOR颗粒数减少,形状及分布趋向正常,与模型组相比差异显著(P<0.01)。结论:免疫营养剂能提高大鼠机体免疫力,调整胃黏膜细胞的增殖,能逆转胃癌癌前病变。

Objective： To investigate the effect of immunonutrition formula precancerous lesions of gastric cancer in rats. Methods： The rats model with precancerous lesions of gastric cancer was established by MNNG, and 90 rats were randomized into experimental group, model group and control group. Animals in model group were fed with common diets, the experimental group were fed with an enteral diet supplemented with immunonutrition formula （120 ml/／[kg／5d／]）. The changes of the gastric mucosa were observed under microscope after 4, 8, and 12 weeks respectively. The levels of blood IgG, IgA, IgM, CD3＋,CD4＋, and CD8＋, as well as AgNOR positive cells counting were examined. Results： In model group, gastric mucosa got thin and atrophied, and intestinal metaplasia were seen, while in experimental group, the mucosa showed no changes. The immune function of rats with precancerous lesions of gastric cancer was inhibited, IgA and IgM levels were significantly reduced, and the ratio of CD4＋/CD8＋ T-cell decreased （all P0.05）. After feeding with immunonutritions for 12 weeks, the level of IgA and IgM resumed normal, the radio of CD4＋/CD8＋ raised, and the AgNOR positive cells counting decreased （all P0.01）. Conclusion： Immunonutritions could effectively reverse the precancerous lesions in gastric carcinoma and its mechanisms of reversion probably relate to the enhance immunological function and nutritional status.