OAP-H_2O_2-HRP伏安酶联免疫分析新体系测定人血清铁蛋白

OAP - H_2O_2 - HRP Voltammetric Enzyme - linked Immunoassay for Trace Ferritin in Human Serum

首次提出邻氨基酚(OAP)-H_2O_2-辣根过氧化物酶(HRP)伏安酶联免疫分析新体系并用于人血清中铁蛋白的测定.本方法以线性扫描二阶导数伏安法检测HRP催化H_2O_2氧化OAP的产物,用于游离HRP和各种HRP标记物的测定,灵敏度均高于经典的ELISA显色光度法,测定游离HRP的线性范围为1.0×10^(-12)～4.0×10^(-9)g/mL,检测限达6.0×10^(-13)g/mL.本法对铁蛋白测定的线性范围为0.2～320ng/ml用所建立的方法对人血清样品进行了测定,并与现行的 ELISA显色光度法进行对照,二者相关性很好.

A voltammetric enzyme - linked immunoassay based on new system of o - aminophenol (OAP) -H2O2 - horseradish peroxidase (HRP) has firstly been developed and used for the detection of HRP and ferritin. HRP or labelled HRP catalyzes the oxidation reaction of OAP with H2O2, producing a sensitive voltammetric peak at - 0.43V (vs. SCE) in Britton - Robinson (B - R) buffer solution. By using this voltammetric peak, HRP can be measured with a detection limit of 6.0 ~ 10-13 g/mL and alinear range of 1.0 × 10-12 ~ 4.0 × 10-9g/mL. The detection limit for ferritin is 0. 2ng/mL and the linear range 0.2 ~ 320ng/mL. The detection limit for ferritin with this method is lower than that with the enzyme- linked immunosorbent spectrophotometric assay (ELISA).