摘要
目的探讨DNA结合抑制蛋白-1(Id-1)基因在腺样囊性癌细胞生长和侵袭行为中的作用。方法以涎腺腺样囊性癌细胞系ACC-M和ACC-2为研究对象,免疫荧光检测Id-1基因的表达;用小干扰RNA进行Id-1基因的RNA干扰;于干扰前后应用RT-PCR和Western blot检测2个细胞系Id-1表达情况;于干扰前后应用MTT法检测细胞的生长情况及用Transwell小室检测细胞的侵袭能力。结果在ACC-M和ACC-2中,Id-1均有表达,ACC-M表达量高于ACC-2;通过RNA干扰沉默Id-1基因后,ACC-M和ACC-2的生长和侵袭能力均受到抑制。在受抑制程度上,ACC-M比ACC-2更为强烈。结论鉴于Id-1对ACC细胞生长和侵袭行为的重要影响,干扰Id-1基因的表达,有望成为治疗腺样囊性癌新的有效方法之一。
Objective To investigate the role of inhibitor of DNA binding-1(Id-1) gene in adenoid cystic carcinoma cell growth and invasion behavior.Methods With salivary adenoid cystic carcinoma cell lines ACC-M and ACC-2,dedected Id-1 gene expression was screened with immunofluorescence assay.After Id-1 mRNA knocking-down using small interfering RNA,RT-PCR and Western blot were used to detect the different expressions before and after interference,and the growth of cells before and after interference was deceted using the MTT assay,and the cell invasion ability was checked with the use of Transwell chamber assay.Results Id-1 were both expressed in the ACC-M and ACC-2,and the expression in ACC-M was higher than that in ACC-2.After Id-1 RNA interference,the growth and invasiveness of ACC-M and ACC-2 were inhibited with the restrained degree in ACC-M much stronger than that in the ACC-2.Conclusion In view of the important role of Id-1 in the behavior of growth and invasion in ACC cell,interfering the expression of Id-1 gene is expected to be a novel and effective means for the treatment of adenoid cystic carcinoma.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2011年第1期66-70,共5页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30672339)
教育部留学回国人员科研启动基金资助项目(2007)
关键词
DNA结合抑制蛋白-1
腺样囊性癌
增殖
侵袭
inhibitor of DNA binding-1
adenoid cystic carcinoma
proliferation
invasion