CDMP和TGF联合诱导BMSCs修复软骨缺损

Repairing defects with bone marrow mesenchymal stem cell inducted by CDMP and TGF-β1

目的探讨骨髓间充质干细胞(BMSCs)在软骨源性形态发生蛋白1(CDMP1)和转化生长因子-β1(TGF-β1)联合诱导前后成软骨能力的差异。方法采用免疫组织化学和阿新蓝染色的方法,检测BMSCs与聚乳酸/羟基乙酸共聚物(PLGA)构筑的三维立体培养体系在CDMP1和TGF-β1联合诱导前后,产生特异性软骨基质Ⅱ型胶原和蛋白多糖(GAG)能力的差异;将诱导前后的培养体系移植入兔甲状软骨全层缺损处,从大体、组织学方面观察其对软骨缺损的修复作用。结果 BMSCs在PLGA上生长良好,CDMP1和TGF-β1诱导后的培养体系可表达Ⅱ型胶原和GAG;将诱导前后的培养体系移植入动物体内,可更加有效地修复喉软骨缺损。结论 BMSCs在CDMP1和TGF-β1联合诱导后比单独应用其中一种修复软骨缺损更加有效。

Objective Clinical application of implanatation of BMSCs in PLGA scafford and induced by cartilage- derived morphogenetic protein 1 （ CDMP1 ） and transforming growth factor-β1 （ TGF-β1 ） to repair defects of cartilage. Methods Under the culture of high-density cell suspension and PLGA frame, BMSCs were observed the ability to repair cartilage combined with PLGA before and after the induction of CDMP and （or） TGF-β1. The complex was implanted into animals and the culturing system was analyzed at the gross level, the histological expression to see the ability to repair cartilage defects. Results The culture system was induced to express cartilagespecific matrix collagen II and GAG. The culturing system could repair cartilage defects effectively after transplanted into animals. Conclusion The BMSCs and PLGA complex induced by CDMP1 and TGF-β1 can repair cartilage defects more effectively than the BMSCs and PLGA complex induced by CDMPI or TGF-β1 only.