摘要
详细研究了等点附近Hg(Ⅱ)-HSA或-BSA 体系的紫外光谱。与生理pH时的谱图相比较,Hg(Ⅱ)-BSA 的250 nm 附近谱带普遍消失或变弱,而290 nm 谱带及Hg(Ⅱ)-HSA的光谱基本不变。这一差异进一步支持了Hg(Ⅱ)离子在HSA 或BSA中最可能的结合位置在7对相邻的二硫桥处,金属中心有四面体型MS4结构的推断。首次提出了四面体型MS4 结构图形,并用配位微环境的差异对结果进行了深入的讨论。
Detailed studies on the UV spectra of Hg(Ⅱ) HSA or Hg(Ⅱ) BSA complexes nearby isoionic point were carried out in this paper.Compared with the spectra at physiological pH,the band nearby 250 nm of Hg(Ⅱ) BSA generally disappeared or widened and weakened,but the band at 290 nm of Hg(Ⅱ) BSA and Hg(Ⅱ) HSA remained nearly unchanged.These results support that these binding sites of Hg(Ⅱ) serum albumin may be located at seven pairs of adjacent disulfide bridges and there might be the structure of HgS 4 tetrahedral configuration.For the first time,this paper put forward the solid figure of HgS 4 tetrahedral configuration,and further discussed the results using the discrepancy of the coordinate micro environment.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
1999年第5期700-703,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金
广西十百千人才基金
关键词
汞
血清白蛋白
结合
配位微环境
结构
Hg(Ⅱ) HSA, Hg(Ⅱ) BSA, UV spectrum, Coordinate micro environment, Structure of metal center