FT-Raman光谱研究溶菌酶在氘代甲醇溶液中的构象变化

The Investigation for Lysozyme Conformation Changes in Deuteromethanol Solution by FT Raman Spectrometer

用ＦＴ－Ｒａｍａｎ光谱研究了在不同浓度的氘代甲醇－重水溶液中溶菌酶的构象。结果表明，随着氘代甲醇浓度的增加，溶菌酶结构逐渐松散，并形成凝胶。松散β－折叠结构增多是产生凝胶的原因。

Lysozyme conformation in deuteromethanol deuteroxide solution has been studied by FT Raman spectrometer.By using amide I mode in the Raman spectra and curve fitting method,information of Lysozyme′s secondary structure is easily detected in details,and tertiary structure changes can be suggested by relative intensity changes of some bands.It is concluded that lysozyme conformation may be changed to loose structure with incrasing deuteromethanol concentration,and it seems like that increasing loose β sheet content lead to the gel′s appearing in the solution.