心肌缺血时尿液中脂肪酸结合蛋白的浓度变化及意义

Changes and Significance of H-FABP in Urine Following Acute Myocardial Ischemia

目的:研究心肌缺血不同的时间段血浆和尿液中心脏型脂肪酸结合蛋白(H-FABP)含量的变化规律,探讨其在早期诊断急性心肌梗死方面的价值。方法:在结扎兔心脏左冠状动脉制作急性心肌缺血动物模型,采用ELISA法检测心肌缺血不同时间段血浆和尿液中H-FABP的浓度。结果:在兔心肌缺血15m in时,血浆和尿液中H-FABP含量即有升高,并且随着心肌缺血时间延长,血浆和尿液中H-FABP的浓度在缺血4h达最高值,与对照组相比差异均有统计学意义。结论:血浆和尿液中H-FABP含量在早期心肌缺血时皆有升高,二者对早期急性心肌梗死都具有诊断价值。

OBJECTIVE：To evaluate the efficacy of heart-type fatty acid binding protein（H-FABP）for early diagnosis of Acute Myocardial Infarction,and to compare the content of heart-type fatty acid binding protein in rabbit plasma and urine at different stages of acute myocardial ischemia.METHOD：The rabbit model of myocardial ischemia was induced by ligating the anterior branch of the left coronary artery,the content of H-FABP in rabbit plasma and urine at different stages of acute myocardial ischemia were detected with the ELISA method.RESULTS： When the myocardial ischemia lasts for 15 min,the H-FABP content in the plasma and the urine namely has elevated.And along with the expansion of myocardial ischemia time,the H-FABP content all gradually advances in the plasma and the urine,the H-FABP density in ischemia for 3 hours reaches highest in the plasma,but the H-FABP density ischemia for 4 hours reach the maximum.There was obvious statistical difference between in the urine ischemia groups and control groups.CONCLUSIONS：Plasma and urine H-FABP levels in early myocardial ischemia are elevated,both can be used in the diagnosis of the early Acute Myocardial Infarction.