铽-EDTA-槲皮素荧光体系及槲皮素的测定

Fluorimetric Study of Tb EDTA Quercetin and Determination of Quercetin

铽－ＥＤＴＡ－槲皮素在碱性条件下生成三元络合物，在紫外光照射下发生分子内能量传递，槲皮素能敏化Ｔｂ３＋的发光，铽的荧光强度与槲皮素的浓度成线性关系。据此建立了一个快速、灵敏、简单的分析血清、尿液和芦丁与槲皮素混合物中槲皮素含量的一种新方法。该方法的线性范围为５．０×－７～１．０×－５ｍｏｌ·Ｌ－１，检测限为５．１×１０－８ｍｏｌ·Ｌ－１，方法成功地应用于未经处理的血清、尿液中槲皮素的测定，其回收率在１０３％～９６％。

Tb EDTA Quercetin complexes were formed under basic conditions.Intramolecular energy transfer occurs in the Tb EDTA complex with quercetin(QCT) and the terbium(Ⅲ) ion fluorescence can be sensitized by QCT.Accordingly a fluorimetric method was used for the determination of QCT in urine,serum and mixed substance of rutin quercetin.The method is simple,sensitive and fast.The linear range is 5 0× -7 1 0×10 -5 mol·L -1 and the detection limit is 5 0×10 -8 mol·L -1 .This method can be applied to the determination of QCT in non pretreated urine and serum successfully.The recoveries are in the range of 103% 96%.