吸光度比值-导数光谱新方法及其在两组分同时测定中的应用

A New Absorbance Ratio Derivative Method and Its Application to Simultaneous Determination of Two Components in a Mixture

本文叙述了吸光度比值－导数光谱新方法的原理，研究了在普通分光光度计上用计算法完成吸光度比值导数运算、同时测定苯酚和间苯二酚含量的实验方法，取得了较为满意的结果。苯酚的回收率为９９．４％～１０４．２％（ｎ＝２５，ＲＳＤ＝１．１％），间苯二酚的回收率为９７．１％～１０２．８％（ｎ＝２５，ＲＳＤ＝１．６％）。

In this paper,the principle of a new absorbance ratio derivative method was described.The experimental methods of derivation with subtraction technique on ordinary spectrophotometer and simultaneous determination of phenol and resorcin were studied.The result is satisfying.The recovery of resorcin and phenol is in the range of 99 4% 104 2% ( n=25 ,RSD=1 1%) and 97 1% 102 8%( n=25 ,RSD=1 6%) respectively.