四川省马铃薯主栽品种的遗传多样性分析

Genetic Diversity of Potato(Solanum tuberosum L.) in Sichuan Province

简单重复序列(SSR)是进行遗传多样性研究的一种有效分子标记。选用四川省现有及引进的马铃薯品系(种)42份,利用已发表的马铃薯SSR引物进行扩增,采用聚类分析方法对供试材料进行了遗传多样性分析。从110对SSR引物中筛选出16对多态性高、扩增稳定、重复性好的引物,16对SSR引物共扩增出130个位点,其中多态性位点116,占总扩增位点的89.2%。利用NTSYS软件进行聚类分析,结果显示四川省栽培的马铃薯品种遗传多样性水平较低,品种间的遗传差异较小。

Simple Sequence Repeat（SSR） was one of the effective techniques for analysis of genetic diversity.42 cultivars in Sichuan province were measured by potato SSR primers published recently.Cluster analysis was used to gain the genetic diversity of the 42 samples.16 pairs of primers had been selected from 110 pairs of primers,because of their high polymorphism,stiff stability,and good repeatability.We identified 130 bands with 16 pairs of primers,and 116 bands were polymorphic,accounting for 89.2 % in the total 130 bands.The result of cluster analysis based on SSR markers showed that there was a low genetic diversity among the major potato cultivars in Sichuan province,and the genetic differences among these samples were also minor.