乙型肝炎病毒DNA复制状态与原发性肝癌的关系研究

The Relationship between HBV-DNA Replication and Primary Hepatic Carcinoma

目的探讨血清乙型肝炎病毒（HBV）DNA复制与原发性肝癌（PHC）的关系。方法选取2008年7月—2009年12月在我院门诊就诊并初次确诊且从未经过抗病毒治疗的PHC患者60例,采用酶联免疫吸附试验双抗体夹心法（ELISA法）检测HBV感染和HCV感染的血清标志物;采用荧光定量聚合酶连反应（PCR法）对HBV感染者行HBV-DNA定量检测。结果 60例PHC患者中,单纯HBV感染者53例（占88.3%）。53例单纯HBV感染的PHC患者中,45例（84.9%）合并肝硬化;血清HBV-DNA阳性率为90.6%（48/53）;HBV-DNA定量〈102cop-ies/ml 5例（占9.4%）,102－104copies/ml者8例（占15.1%）,〉104copies/ml者40例（占75.5%）。结论大部分PHC患者存在HBV感染,且随着病毒载量的上升,PHC的构成比上升。可见,HBV感染与PHC发病有关,且PHC的发生与HBV-DNA活动性复制呈正相关。

Objective To explore the relationship between viral（HBV） replication level and the pathogenesis of primary hepatic carcinoma（PHC）.Methods 60 patients admitted in our hospital from July 2008 to December 2009 were selected.Those patients were firstly diagnosed with PHC in our hospital and have not been treated by anti-virus therapy.HBV infection and HCV infection were detected by enzyme-linked ELISA,and HBV-DNA quantification was detected by PCR.Results In the 60 patients with PHC,53 cases（88.3%） had HBV infection alone,among which 45 cases（84.9%） were with cirrhosis,the positive rate of serum HBV-DNA was 90.6%,as concerns the patients′ HBV-DNA quantification,40 cases（77.5%） were higher than 104 copies/ml,8 cases（15.1%） were between 102 and 104 copies/ml,and 5 cases（9.4%） were lower than 102 copies/ml.Conclusion It is confirmed that HBV infection is a major starting factor to PHC,and the more the HBV-DNA load is,the higher the incidence of PHC will be.The occurrence of PHC has a positive correlation with HBV-DNA replication.