血红蛋白在L-半胱氨酸微银修饰电极上的电化学行为

Electrochemical Behavior of Hemoglobin at a L-cysteine Modified Microelectrode

L-半胱氨酸通过化学键结合到微银电极表面成化学修饰电极,研究表明此修饰电极对血红蛋白的氧化还原有促进作用.并用预富集和示差脉冲溶出伏安法检测血红蛋白的浓度,检测限为1.0×10^(-8)mol/L,8次测定2.0×10^(-7)mol/L血红蛋白的相对标准偏差是6.3%.

L-cysteine is covalently combined onto a silver microelectrode to form a chemically modified electrode. Experimental results show that hemoglobin possesses good voltammetric response at this electrode. Preconcentration and differential pulse cathodic stripping voltammetry are used to detecthemoglobin. The relative standard deviation is 6.3% for 8 successive determinations at 2.0×10-7mol/LHemoglobin. The detection limit is down to 1.0×10-8mol/L.