水解酶模型分子的光谱特性研究

Research of Spectra Characterization of Hydrolutic Enzymes Model Molecule

研究具有催化活性的水解酶模型分子：三－（2－苯并咪唑甲基）胺（tris（2－benzimidazylmethyl）amine）与Cu（Ⅱ）、Co（Ⅱ）、Zn（Ⅱ）三种金属离子配位形成的配合物的紫外可见吸收光谱和荧光光谱。分析了MTB系列配合物的光谱的电子跃迁，计算了不同pH值时的荧光量子产率。通过分析不同金属离子配合物及不同pH值时荧光量子产率和催化水解活性的关系，发现NTB系列配合物随着催化活性的增加，荧光量子产率有所降低的规律．

The fluorescence and electornic absorption speetra of (tris(2 - benzimidazylmethyl )amine and its Cu (Ⅱ), Co (Ⅱ), Zn (Ⅱ) complexes that can catalyze hydrolytic have been studied.The electrnic thensitions of NTB and its mdel complexes wer analynd, the absorption bands in a range of 270 ~ 290nm and at about 300nm can be assigned to π→π and n →πrespectively. The quantum yields were calculated . The quantum yields were caculated . The quantum yields of the NTB -Zn was much higher than that of the NTB - Cu and NTB - Co.The hydrolytic catalyzing rate of NTB -Cu and NTB -Co wer higher than that of NTB -Zn.It is shown that the hydrolytic catalyzing rate decreased with the increase of the fluorescence quantum yield. The hydrolytic catalyzing rates of Cu(Ⅱ), Co(Ⅱ), Zn(Ⅱ ) complexes were reduced when the pH values were raised. And the fluorescence quantum yields were raised when the pH values wer raised. It's testified that the conclusion were right further.