锌试剂与牛血清白蛋白作用机理的研究

Studies on the Reaction Between Zincon and Bovine Serum Albumin

采用ＵＶ光谱法研究了锌试剂与牛血清白蛋白（ＢＳＡ）在弱酸性溶液中的结合反应， 研究了溶液吸光度与ＢＳＡ 浓度的关系． 测得其表观摩尔吸光系数εＰ＝ １．３×１０６ Ｌ·ｍ ｏｌ－ １·ｃｍ － １， 最大结合数ｎ＝ ２７８， 表观结合常数Ｋｃ＝ ８×１０７． 研究了小分子探针与蛋白质的反应机理及在蛋白质上的结合部位及结合力类型． 它们之间主要是以分子间的静电引力结合反应． 离子强度对结合反应有显著的影响； 不同类型的表面活性剂均以不同的程度和形式对反应有影响． 讨论了此结合反应的模式， 认为该反应基本符合Ｓｃａｔｃｈａｒｄ 模型．

The interaction of zincon(ZCN) with bovine serum albumin(BSA) and the changes of its spectrum were studied by UV spectra at pH 4 1 buffer solution. The conditional constants, apparent molar absorptivity ε P=1 3×10 6 L·mol -1 ·cm -1 , maximum binding number n =278 and apparent binding equilibrium constant K c=8×10 7 were obtained. It was considered that electrostatic force is the main binding force. The ion(sodium chloride) concentration of the solution has significant effect on binding reaction of BSA and ZCN. The effects of different denaturants were also examined. The Scatchard model is appropriate in the treatment of data obtained.