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延边黄牛体细胞克隆早期胚胎端粒酶活性的测定 被引量：2

Determination of Telomerase Activity in Early Embryo Cloned from Somatic Cells of Yanbian Cattle
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摘要 为了解延边黄牛体细胞克隆早期胚胎发育过程中端粒酶活性的变化规律,采用实时荧光定量PCR法检测体细胞克隆早期胚胎及卵母细胞端粒酶活性。研究结果表明,端粒酶活性在未成熟卵母细胞相对水平较高,2-细胞至16-细胞胚胎的端粒酶活性总体水平相对较低(P<0.05),桑椹胚的端粒酶活性总体水平显著升高(P<0.05),在囊胚阶段达到最高。 The objective of this study was to examine the telomerase activity in early embryo cloned from somatic cells of Yanbian cattle by real-time PCR.The results were as follows：the telomerase activity in immature oocyte was higher,that of 2-cell^16-cell is lower than that of the others（P0.05）. Telomerase activity increased significantly in the morula stage （P0.05）,the highest was in the blastocyst stage.
作者 张宇 徐颖 吴昊 方南洙 李钟淑
机构地区 延边大学农学院
出处 《江西农业大学学报》 CAS CSCD 北大核心 2010年第6期1236-1239,共4页 Acta Agriculturae Universitatis Jiangxiensis
基金 国家自然科学基金项目(30860184) 振兴东北老工业基地项目(发改高技[2004]2057号) 延边州延边大学合作项目(延大科合字(2005)06号)
关键词 实时定量PCR 早期胚胎 端粒酶活性 real-time PCR early embryo telomerase activity
分类号 S823.811.2 [农业科学—畜牧学]
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参考文献11

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同被引文献54

  • 1张静,郑月慧,郑丽萍.排卵前期卵泡颗粒细胞端粒酶的表达及其影响因素[J].生理学报,2005,57(6):714-718. 被引量：5
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江西农业大学学报
2010年 第6期

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