绿豆幼苗萌发期的超弱发光检测

Detection of Ultraweak Biophoton Emission during Mung Bean Seedling

采用以EMCCD(电子倍增式CCD)为主的、自行搭建的超弱光图像探测系统测得绿豆幼苗在萌发期间的自发超弱发光和白光照射后的诱导发光(延迟发光).结果表明,绿豆幼苗萌发期的自发发光与延迟发光的强度及其时间分布有明显区别:自发发光强度远小于延迟发光强度;与有丝分裂和生长代谢有关的超弱发光在光诱导下得以加强;此外,数据非线性拟合发现绿豆幼苗的发光强度-时间曲线遵循指数衰减规律,说明生物光子具有一定的相干性.

Using an ultraweak luminescent image detection system, which is composed of detector-electronmultiplier CCD camera （EMCCD）the ultraweak photon emission of mung bean seedling was detected. We found that the intensity and distribution of spontaneous bioluminescence （SL） were different from that of delayed bioluminescence（DL） induced by white light illumination. Generally, the intensity of SL is lower than that of DL, demonstrating that the ultraweak bioluminescence related to the mitosis and metabolism of the sample was strengthened after the sample was illuminated by white light. In addition, the measured photon intensity-time curve follows an exponential decay law, indicating the coherence of biophoton emission.