Caco-2细胞模型比较黄芩苷和黄芩苷滴丸在小肠的吸收

Comparison of the small intestinal absorption of baicalin and baicalin drop pills by Caco-2 cell model

目的:比较黄芩苷和黄芩苷滴丸在小肠的吸收。方法:采用Caco-2细胞单层模型研究黄芩苷和黄芩苷滴丸由绒毛面到基底面的跨膜转运过程。通过测定黄芩苷和黄芩苷滴丸在Caco-2细胞模型的转运百分率及表观渗透系数(Papp),比较二者的跨膜转运能力。结果:在细胞转运实验中,135min时,黄芩苷和黄芩苷滴丸的转运百分率分别为1.00%和6.25%,Papp分别为(0.664±0.103)×10-6cm·s-1和(4.462±1.10)×10-6cm·s-1,黄芩苷滴丸的Papp是黄芩苷的6倍。在跨膜转运180min内,黄芩苷滴丸的转运百分率与时间成正比。结论:将黄芩苷制成滴丸可能提高黄芩苷在小肠的吸收。

Objective：To study the absorption of baicalin and baicalin drop pills through small intestine.Methods：Using Caco-2 cell monolayers as an intestinal epithelial cell model,transmembrane transports of baicalin and its drop pills were studied from apical side（AP） to basolateral side（BL）.The ability of transmembrane transport was compared between the baicalin and its drop pills by determining the transfer percentage and the apparent permeability coefficient（Papp） in the Caco-2 cell model.Results：After transport of 135 minutes,the transfer percentage of baicalin and baicalin drop pills were 1.00% and 6.25% respectively,and the Papp values（from AP to BL） were（0.664±0.103）×10-6 cm·s-1 and（4.462±1.10）×10-6 cm·s-1 respectively.The Papp value of baicalin drop pills was about six times higher than that of baicalin.The transfer percentage of baicalin drop pills was proportional to time within 180 minutes.Conclusion：The absorption of baicalin through small intestine may be significantly improved in baicalin drop pills.