不同治疗时间法舒地尔对心力衰竭大鼠心肌细胞内钙离子浓度的影响

Impact of calcium concentration in myocardial cells of the rat with heart failure in treatment with fasudil in different treatment time

目的:探讨不同治疗时间法舒地尔(fasudil)对心力衰竭大鼠心肌细胞内钙离子浓度的影响。方法:将升主动脉缩窄术后心力衰竭Wistar雌性大鼠随机分为4组,每组10只,两组为心力衰竭组,给予0.9%NaCl溶液,0.1ml,腹腔注射,每日2次;另两组给予fasudil5mg/kg,腹腔注射,每日2次(n=10);分别治疗2周或6周。同时制备两组机械性套环模拟组模型作为对照组(n=10),不予处置。观察每组大鼠各项指标变化。结果:心力衰竭鼠心肌细胞钙离子浓度([Ca2+]i)显著增高,fasudil组随干预时间增加改善心力衰竭症状更明显,但心肌细胞内[Ca2+]i无明显改变。结论:法舒地尔能随干预时间的延长而有效的缓解心力衰竭症状,但不降低细胞内[Ca2+]i,可能为一种新的、有效的治疗心力衰竭的血管扩张剂。

Objective：To investigate impact of calcium concentration in myocardial cells of the rat with heart failure in treatment with fasudil in different treatment time.Methods：Female Wistar rats after ascending aorta coarctation operation were divided into four groups,10 every group,two groups of heart failure were treated with the physiological saline,0.1 ml,abdominal injection,2 times per day;two groups were treates with fasudil 5 mg/kg,abdominal injection,2 times per day（n=10）;treatment of two weeks or six weeks.At the same time produced a set of machinery two analog groups model as a control group（n=10） and did not be disposed.The indexes of all groups were observed.Results：Calcium concentration in myocardial cells of the rat with heart failure（[Ca2＋]i） had a significant increase,with the intervening period increasing,heart failure symptoms of fasudil group improved more obvious,but the heart muscle cells [Ca2＋]i had no significant change.Conclusion：With the intervening period increasing,the symptoms of heart failure is effectively alleviate,but cells [Ca2＋]i is not lower,it could be a new,effective treatment of heart failure in the expansion of blood vessels.