高通滤波器在荧光分子显微中的超分辨

SUPERRESOLUTION IN THE FLUORESCENT MOLECULE MICROSCOPY USING HIGH-PASS FILTERS

荧光局域显微是在倏逝场作用下荧光分子发光并成像.作者应用矢量衍射理论分析了高通滤波器对荧光局域显微镜的优化作用,包括光斑大小、光斑的强度和光斑的边瓣强度,研究了成像参数与高通滤波器的区域边界参数之间的关系.数值结果指出,具有适当区域边界的高通滤波器对荧光分子显微可实现超分辨,并且荧光分子所在的样本层的折射率愈大,超分辨效果愈明显.

Fluorescence localization microscopy is a technology that during the action of evanescent filed fluorescence molecules emit fluorescence and then image.In this paper,the optimizing functions（to the spot size,the intensity of spot and the sidelobe intensity of spot） of high-pass filters to fluorescence localization microscopy are analyzed using the vector diffraction theory.The relationship between imaging parameters and the boundary parameter of the high-pass filter is studied.Numerical results show that some high-pass filters with proper boundary can achieve superresolving effect on fluorescence microscopy.The larger the refractive index of sample layer which fluorescence molecules locate in is,the more remarkable the superresolution effect is.