摘要
在模拟生理环境的条件下,通过不同的光谱方法(紫外、荧光和圆二色谱等)来检测药物枸橼酸他莫昔芬分别与鲑鱼精DNA和牛血清白蛋白的相互作用。根据实验数据分别计算出了枸橼酸他莫昔芬与DNA和BSA的动态淬灭常数KSV、kq以及缔合位点n。因为药物与DNA的缔合常数小于药物与BSA的缔合常数,所以相对于DNA来说,药物更容易与蛋白质结合;枸橼酸他莫昔芬与DNA和BSA的缔合位点均在1左右,说明药物与DNA和BSA的缔合方式只有1种;同时,定性得出药物与DNA的相互作用为嵌插缔合。通过热力学常数的计算,得出药物和BSA的相互作用为疏水作用。
Interaction of tamoxifen citrate （TC） with salmon sperm DNA and bovine serum albumin （BSA） were investigated by various spectroscopic analysis methods under simulative physiological conditions. Thermodynamic parameters showed that the interaction process was spontaneous and hydrophobic forces play a major role in the binding between BSA and TC. The quenching constant KSV,the bimolecular quenching constant kq and the numbers of binding sites n （about 1） of both DNA and BSA to TC were similar to each other. The value of n approximately equal to 1 indicated that there is only one type of binding site for DNA or BSA to TC. However,the binding constant （K） of DNA to TC was lower than that of BSA.
基金
国家自然科学基金资助项目(20775029,J0730425)
教育部新世纪优秀人才支持计划资助项目(NCET-07-0400)
兰州大学骨干教师资助项目
