生长因子对间皮细胞增殖及合成细胞外基质的影响

Effect of growth factors on proliferation and extracellular matrix biosynthesis by human peritoneal mesothelial cells

目的 探讨血小板源生长因子ｂｂ（ＰＤＧＦｂｂ） 、转化生长因子β（ＴＧＦβ） 对人腹膜间皮细胞（ＨＰＭＣ）增殖及合成细胞外基质（ＥＣＭ） 的影响。方法 建立ＨＰＭＣ体外培养体系。３ＨＴｄＲ 掺入法测定细胞增殖；放射免疫法检测细胞上清中Ⅲ型前胶原（ ⅢＰＣ） 。结果 ＰＤＧＦｂｂ 促进、但ＴＧＦβ抑制ＨＰＭＣ增殖，均呈剂量依赖性（ Ｐ＜ ０－０１） 。２ 者均能刺激ＨＰＭＣ 产生ⅢＰＣ（ Ｐ＜ ０－０１）。结论 ＣＡＰＤ相关性腹膜炎时腹腔中高浓度的ＰＤＧＦｂｂ 、ＴＧＦβ能调节间皮层的修复和重塑，刺激其合成分泌ＥＣＭ，后者可能是腹膜炎（ＰＩ）反复发生最终导致腹膜硬化的重要机制之一。

Objective To examine the effect of PDGF bb and TGF β on the proliferation and extracellular matrix(ECM) biosynthesis by human peritoneal mesothelial cells (HPMC).Methods In vitro HPMC culture system was established. Cell proliferation was assessed by 3 H TdR incorporation and type Ⅲ precollagen (ⅢPC) in supernatants was quantitated by radioimmunoassay. Results PDGF bb stimulated, but TGF β inhibited the proliferation of HPMC in dose dependent manner (P<0 01). Both growth factors induced significant increases in ⅢPC production by HPMC (P<0 01).Conclusion During peritonitis complicated with CAPD, enhanced PDGF bb and TGF β in the peritoneal cavity may regulate the repairing and remodeling of mesothelium, and accelerate the synthesis of ECM by HPMC, which might potentially contribute to the pro fibrotic process in CAPD.