摘要
采用高效液相色谱法建立了虫草黄酒中虫草素含量的测定方法,探讨了虫草素提取条件,分析了流动相组成、柱温、波长对检测结果的影响.研究表明:色谱柱为Waters Symmetry C18柱,流动相为甲醇∶水=15∶85,流速为1mL/min;检测波长为288nm,柱温为40℃时分离、检测效果最好;方法检出限为0.05ng.该测定方法在黄酒质量控制方面具有应用价值.
A method to determine 3＇-deoxyadenosine in Cordyceps sinensis yellow wine has been established with high performance liquid chromatography.The effect of several factors were studied,such as type of component of mobile phase,column temperature,detective wave length and the condition of extracting the 3＇-deoxyadenosine.The studies indicate that the effect of separation and determination are optimum when determination parameters are as follows：mobile phase：methanol/water=15/85;flow rate：1 mL/min;detective wave length：288 nm;column temperature：40 ℃.The detection limit is 0.05 ng.This detection method is of practical use in the quality control of yellow wine.
出处
《徐州工程学院学报（自然科学版）》
CAS
2010年第4期77-80,共4页
Journal of Xuzhou Institute of Technology(Natural Sciences Edition)
基金
浙江省质量监督局计划项目(20100228)
