中国云南省五个民族DYS287位点多态性的调查

Investigation of Y chromosome and polymorphism of 5 Chinese ethnic groups in Yunnan province,China

目的 对中国云南的汉族、傣族、拉祜族、佤族、藏族5 个群体的DYS287 位点多态性进行筛查。方法 利用PCR法对此位点进行扩增,经过琼脂糖凝胶电泳检测结果。结果 汉、傣、拉祜、佤族均为YAP- ,扩增片段均为 150bp,而 30 个藏族人中有11 个为 YAP+ ,扩增片段为 455bp,YAP+ 频率达36.7% 。结论 DYS287 位点可以作为一种重要、稳定的遗传标记对遗传多样性研究提供可靠的证据。

Objective To investigate the polymorphism of DYS287 in the Han,Dai,Lahu,Wa and Tibetan groups living in Yunnan province,China.Methods YAP element was detected by PCR amplifica tion and agarose gel electrophoresis.Results With 150bp product in all Han,Dai,Lahu and Wa individuals,YAP element is absent.In 11 Tibetan individuals out of 30,YAP element is present and the PCR product is 455bp.Conclusion As an important and stable genetic marker,DYS287 can provide reliable evidence in evolution study.