霍乱弧菌及其他致病弧菌DNA多态性的研究

DNA polymorphism of Vibrio Cholera and other Vibrios

目的 探讨霍乱弧菌和其它致病弧菌分子遗传特征及相互关系。方法 用聚合酶链反应(PCR)、DNA序列分析、随机扩增多态性DNA(RAPD)和平均链锁聚类分析 ,对 3株O1群古典型、4株E1Tor型、3株O13 9群霍乱弧菌和副溶血等 3株致病弧菌进行检测。结果 O13 9群霍乱弧菌和O1群含有相同的霍乱肠毒素 (CTX)基因 ,核苷酸序列同源性为 96 9%～ 98 7%。其它致病弧菌不含有CTX基因。RAPD将不同弧菌分成 4类 ;即古典型、E1Tor型和O13 9群、副溶血和创伤弧菌及河弧菌。O13 9群与O1群E1Tor型DNA指纹图谱几乎完全一致 ,平均链锁距离为 0 ,与古典型相似 ,平均链锁距离为 2 0 7。与副溶血等致病弧菌差异较大 ,平均链锁距离为 6 76～ 8 5 4。结论 霍乱弧菌和其它致病弧菌遗传特征表现出多态性。O13 9群霍乱弧菌与E1Tor型遗传特征相同 ,前者很可能由E1Tor型进化而来 ,副溶血与创伤弧菌也具有相同特征。

Objective To investigate the inherent characteristics of V.cholera and other vibrios and the association between them. Methods Polymerase chain reaction (PCR), DNA sequences analysis, randomly amplified polymorphic DNA (RAPD) and average linkage cluster analysis were used to study V. cholera including 3 strains of O139, 4 strains of E1 Tor biotype, 3 strains of classical and 3 other vibrios. Results V. cholera O139 contained the genomic sequences of CTX A2 B as well as O1 V. cholera , indicating that their homologous nucleotidies was 96.9%～98.7%, and other vibrios did not contain CTX. O1 V. cholera and other vibrios were divided into 4 groups by RAPD: classical; O139 and E1 Tor; V. paraheamolyticus and V. vulnificus ; and V. flluvialis . The O139 DNA fingerprints of RAPD were almost as same as E1 Tor biotype, whose average linkage cluster distance was 0, similar with classical biotype, whose average linkage cluster distance was 2.07, much different from V. paraheamolyticus and others with the average linkage cluster distance being 6.76～8.54. Conclusion V.cholera and other vibrios are polymorphic in inherent characteristics. Being as same as the inherent characteristics of E1 Tor, O139 may evoluted from E1 Tor biotype. And also the inherent characteristics of V.parahemolytisus were as same as those of V.vulnificus .