摘要
以吖啶橙(AO)为探针,在Tris-HCl(pH=7.40)缓冲溶液中,采用荧光光度法及黏度法研究了偶氮胂Ⅲ与DNA相互作用的机理。结果发现,偶氮胂Ⅲ与DNA的作用方式为部分插入模式,摩尔结合比为2 1,25℃时的结合常数为4.10×106L/mol。通过热力学研究认为偶氮胂Ⅲ与DNA的相互作用为熵驱动过程。
The interaction of the ASAⅢ with herring sperm DNA has been studied by fluorescence and viscosity methods in physiological pH environment（pH=7.40） with AO as the spectral probe.The results show that the interaction modes between ASAⅢ and DNA are partial and nonclassical mixed intercalation.The binding ratio of nASAⅢ：nDN A=2：1,and the binding constants is KΘ25 ℃=4.10×106 L/mol.The interaction between ASAⅢ and DNA is an enthalph driven reaction.
出处
《西南科技大学学报》
CAS
2010年第4期9-12,共4页
Journal of Southwest University of Science and Technology
基金
国家高技术研究发展计划(863计划)资助(2007AA×××133)
国家自然科学基金资助(30572254)
