不同类型应激对大鼠神经和免疫源降钙素基因相关肽释放的影响

Effect of different stress on nerve or immunal derived calcitonin gene related peptide release in the rat

目的：比较大鼠精神性（ 束缚） 和生物性（ 脓毒败血症） 应激时不同组织中降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅｒｅｌａｔｅｄ ｐｅｐｔｉｄｅ，ＣＧＲＰ）含量的变化。方法：盲肠结扎并以１８ 号针穿刺，制备１０ ｈ 和２０ ｈ 大鼠腹膜炎脓毒血症模型。将大鼠固定２０ ｈ 制备束缚应激模型，以放免法测定６ 种组织（ 细胞） 中ＣＧＲＰ 的含量。结果：腹膜炎脓毒血症早期时，脊髓背根神经节、肠系膜上动脉和十二指肠中ＣＧＲＰ明显升高，晚期时胸腺细胞中ＣＧＲＰ明显升高，气管中ＣＧＲＰ持续升高，心房中ＣＧＲＰ含量呈增高趋势。而束缚应激时仅胃肠道和肠系膜上动脉ＣＧＲＰ含量有明显改变。结论：在不同应激刺激下，神经源和免疫源ＣＧＲＰ 的合成和释放的特点不同。精神应激可能不直接刺激ＣＧＲＰ释放，后者对感染、失血和创伤等刺激更为敏感。

Objective: To compare the calcitonin gene related peptide (CGRP) contents of different tissues following psychogenic (restraint) stress and biological (sepsis) stress. Methods: Rat septic model was made by cecal ligation and puncture with a 18 gauge needle for 10 and 20 hours. Rat restraint stress was made by restricting movement of the rat for 20 hours. The CGRP like immunoactivity in six kinds of tissue/cells was determined by CGRP specific RIA. Results: The data showed that the CGRP contents in dorsal root ganglia, superior mesenteric arteria and duodena were just enhanced significantly in early sepsis, but CGRP was increased in thymocytes and sustained at high levels in tracheas in late sepsis, and it showed a tendency to elevate in atria. In contrast, gastrointestinal and superior mesenteric arterial CGRP content was altered only in the restraint stress. Conclusion: Different stresses induce synthesis and release of nerve and immuno derived CGRP with different characteristics. Psychogenic stress might not directly stimulate CGRP release which is more sensitive to infective, ischemic and traumatic stimuli.