缺血预处理对肾脏内源性保护作用的机制探讨

The role of antioxidant activity in the endogenous protective mechanisms of ischemic preconditioning to the kidney

目的 :通过建立缺血再灌注损伤的动物模型探讨抗氧化能力在缺血预处理对肾脏内源性保护机制中的作用。方法 :54只 SD大鼠随机分为三组 :缺血预处理组、单纯缺血再灌注组和假手术对照组。术后 2 4、72、168小时分三批处死动物、取肾脏组织测定其超氧化物歧化酶 (SOD)、脂质过氧化物 (LPO)和一氧化氮(NO)的含量。结果 :缺血预处理可增加肾组织超氧化物歧化酶 (SOD)合成而减少脂质过氧化物 (LPO)和一氧化氮 (NO)的产生。结论 :抗氧化剂能力可能在缺血预处理对肾脏的内源性保护机制中起重要作用。

Purpose:To observe the effects of Antioxidant activity in the endogenous protective mechanisms of ischemic preconditioning to the kidney by establishing animal model of ischemia-reperfusion injury Materiais and methods:Fifty-four SD rats were divided into 3 groups,group 1 was given ischemic preconditioning prior to renal ischemia-reperfusion,group 2 just received ischemia-reperfusion and the third group with sham operation served as control The animals were sacrificed at 24,72,and 168 hours following the operation by three times Superoxide dismutase(SOD),lipid peroxidation(LPO)and nitric oxide (NO)contents in the kidney tissue was measured Results:lschemic preconditioning can improve the antioxidant activity of kidney tissue following ischemia-reperfusion by increasing SOD and reducing LPO and NO in the kidney tissue Conclusions:Antioxidant activity may play an important role in the endogenous protective mechanisms of ischemic preconditioning to the kidney ischemia-reperfusion injury